Marzol E, Borassi C, Carignani Sardoy M, Ranocha P, Aptekmann AA, Bringas M, Pennington J, Paez-Valencia J, Martínez Pacheco J, Rodríguez-Garcia DR, Rondón Guerrero YDC, Peralta JM, Fleming M, Mishler-Elmore JW, Mangano S, Blanco-Herrera F, Bedinger PA, Dunand C, Capece L, Nadra AD, Held M, Otegui MS, Estevez JM. Class III Peroxidases PRX01, PRX44, and PRX73 Control Root Hair Growth in Arabidopsis thaliana. Int J Mol Sci. 2022 May 11;23(10):5375. doi: 10.3390/ijms23105375.

Zheng P, Zheng C, Otegui MS, Li F. Endomembrane mediated-trafficking of seed storage proteins: from Arabidopsis to cereal crops. J Exp Bot. 2022 Mar 2;73(5):1312-1326. doi: 10.1093/jxb/erab519.

Ding X, Zhang X, Paez-Valencia J, McLoughlin F, Reyes FC, Morohashi K, Grotewold E, Vierstra RD, Otegui MS. Microautophagy Mediates Vacuolar Delivery of Storage Proteins in Maize Aleurone Cells. Front Plant Sci. 2022 Feb 18;13:833612. doi: 10.3389/fpls.2022.833612.

Weiner, E., Pinskey, J. M., Nicastro, D., & Otegui, M. S. (2022). Electron microscopy for imaging organelles in plants and algae. Plant Physiology188(2), 713-725.

Birnbaum KD, Otegui MS, Bailey-Serres J, Rhee SY. The Plant Cell Atlas: focusing new technologies on the kingdom that nourishes the planet. Plant Physiol. 2022 Feb 4;188(2):675-679.


Zheng P, Zheng C, Otegui MS, Li F. Endomembrane Mediated-Trafficking Of Seed Storage Proteins: From Arabidopsis To Cereal Crops. J Exp Bot. 2021 Nov 30:erab519.

Kim JH, Lee HN, Huang X, Jung H, Otegui MS, Li F, Chung T. FYVE2, a phosphatidylinositol 3-phosphate effector, interacts with the COPII machinery to control autophagosome formation in Arabidopsis. Plant Cell. 2022 Jan 20;34(1):351-373.

Kang BH, Anderson CT, Arimura SI, Bayer E, Bezanilla M, Botella MA, Brandizzi F, Burch-Smith TM, Chapman KD, Dünser K, Gu Y, Jaillais Y, Kirchhoff H, Otegui MS, Rosado A, Tang Y, Kleine-Vehn J, Wang P, Zolman BK. A glossary of plant cell structures: Current insights and future questions. Plant Cell. 2022 Jan 20;34(1):10-52.

Roeder AHK, Otegui MS, Dixit R, Anderson CT, Faulkner C, Zhang Y, Harrison MJ, Kirchhelle C, Goshima G, Coate JE, Doyle JJ, Hamant O, Sugimoto K, Dolan L, Meyer H, Ehrhardt DW, Boudaoud A, Messina C. Fifteen compelling open questions in plant cell biology. Plant Cell. 2022 Jan 20;34(1):72-102.

Plant Cell Atlas Consortium, Jha SG, Borowsky AT, Cole BJ, Fahlgren N, Farmer A, Huang SC, Karia P, Libault M, Provart NJ, Rice SL, Saura-Sanchez M, Agarwal P, Ahkami AH, Anderton CR, Briggs SP, Brophy JA, Denolf P, Di Costanzo LF, Exposito-Alonso M, Giacomello S, Gomez-Cano F, Kaufmann K, Ko DK, Kumar S, Malkovskiy AV, Nakayama N, Obata T, Otegui MS, Palfalvi G, Quezada-Rodríguez EH, Singh R, Uhrig RG, Waese J, Van Wijk K, Wright RC, Ehrhardt DW, Birnbaum KD, Rhee SY. Vision, challenges and opportunities for a Plant Cell Atlas. Elife. 2021 Sep 7;10:e66877.

Ding X, Zhang X, Olsen OA, Otegui MS. Transient expression of fluorescently tagged proteins in developing maize aleurone cells. MethodsX. 2021 Jul 7;8:101446.

Dorone Y, Boeynaems S, Flores E, Jin B, Hateley S, Bossi F, Lazarus E, Pennington JG, Michiels E, De Decker M, Vints K, Baatsen P, Bassel GW, Otegui MS, Holehouse AS, Exposito-Alonso M, Sukenik S, Gitler AD, Rhee SY. A prion-like protein regulator of seed germination undergoes hydration-dependent phase separation. Cell. 2021 Aug 5;184(16):4284-4298.e27.

Yanagisawa M, Poitout A, Otegui MS. Arabidopsis vascular complexity and connectivity controls PIN-FORMED1 dynamics and lateral vein patterning during embryogenesis. Development. 2021 Jul 1;148(14).

Goodman K, Paez-Valencia J, Pennington J, Sonntag A, Ding X, Lee HN, Ahlquist PG, Molina I, Otegui MS. ESCRT components ISTL1 andLIP5 are required for tapetal function and pollen viability. Plant Cell. 2021 Aug 31;33(8):2850-2868.

Peer WA, Hosein FN, Bandyopadhyay A, Makam SN, Otegui MS, Lee GJ, Blakeslee JJ, Cheng Y, Titapiwatanakun B, Yakubov B, Bangari B, Murphy AS. Corrigendum to: Mutation of the membrane-associated M1 protease APM1 results in distinct embryonic and seedling developmental defects in Arabidopsis. Plant Cell. 2021 Aug 13;33(7):2506.

Liu C, Zeng Y, Li H, Yang C, Shen W, Xu M, Xiao Z, Chen T, Li B, Cao W, Jiang L, Otegui MS, Gao C. A plant-unique ESCRT component, FYVE4, regulates multivesicular endosome biogenesis and plant growth. New Phytol. 2021 Jul;231(1):193-209.

Chacko JV, Lee HN, Wu W, Otegui MS, Eliceiri KW. Hyperdimensional Imaging Contrast Using an Optical Fiber. Sensors (Basel). 2021 Feb 9;21(4):1201.


Paez-Valencia J, Otegui MS. Cell-Free Protein Translation System for Expression of Lipid-Binding Proteins Tagged with Small epitopes and Their Use in Protein-Lipid Overlay Assays. Methods Mol Biol. 2020;2177:143-152.

Czymmek K, Sawant A, Goodman K, Pennington J, Pedersen P, Hoon M, Otegui MS. Imaging Plant Cells by High-Pressure Freezing and Serial block-face scanning electron microscopy. Methods Mol Biol. 2020;2177:69-81.

Otegui MS. Electron Tomography and Immunogold Labeling as Tools to Analyze De Novo Assembly of Plant Cell Walls. Methods Mol Biol. 2020;2149:365-382.

McLoughlin F, Marshall RS, Ding X, Chatt EC, Kirkpatrick LD, Augustine RC, Li F, Otegui MS, Vierstra RD. Autophagy Plays Prominent Roles in Amino Acid, Nucleotide, and Carbohydrate Metabolism during Fixed-Carbon Starvation in Maize. Plant Cell. 2020 Sep;32(9):2699-2724.

Jiang N, Gutierrez-Diaz A, Mukundi E, Lee YS, Meyers BC, Otegui MS, Grotewold E. Synergy between the anthocyanin and RDR6/SGS3/DCL4 siRNA pathways expose hidden features of Arabidopsis carbon metabolism. Nat Commun. 2020 May 15;11(1):2456.

Otegui, M.S. 2020. Imaging polyphenolic compounds in plant tissues. Recent Advances in Polyphenol Research, invited review, in press.

Zhang, X., Ding, X. Marshall, R., Paez-Valencia, J., Lacey, P., Vierstra, R., Otegui, M.S. 2020. Reticulon proteins modulate autophagy in maize endosperm. eLife pii: e51918.

Kendle, R. and Otegui, M.S. 2020. Membrane trafficking and plant signaling. Progress in Botany 81:103-124.

Otegui, M.S. Immunolabeling and electron tomography of plant membrane systems. Methods in Cell Biology, in press.

Czymmek, K., Sawant, A., Goodman, K., Pennington, J., Pedersen, P., Hoon, M., Otegui, M.S. 2020. Imaging plant cells by high-pressure freezing and serial block-face scanning electron microscopy. Methods in Molecular Biology, in press.

Paez-Valencia, J. and Otegui, M.S. 2020. Cell-free protein translation system for expression of lipid-binding proteins tagged with small epitopes and their use in protein-lipid overlay assays. Methods in Molecular Biology, in press.

Otegui, M.S. 2020. High-pressure freezing and freeze substitution for transmission electron microscopy imaging and immunogold-labeling. Methods in Molecular Biology, in press.

Otegui, M. (Editor). 2020. Plant Endosomes, Methods in Molecular Biology, 2nd edition, Humana Press. In press.


Tsednee, M., Castruita, M., Salomé, P.A., Sharma, A., Lewis, B.E., Schmollinger, S.R., Holbrook, K.N., Otegui, M.S., Khatua, K., Das, S., Datta, A., Chen, S., Ramon, C., Ralle, M., Weber, P.K., Stemmler, T.L., Pett-Ridge, J., Hoffman, B.M., Merchant, S.S. 2019. Hyper-accumulated Mn, co-localized in acidocalcisomes with Ca and P, can be mobilized in Mn-deficient situations. J. Biol. Chem 294:17626-17641. PMID 31527081.

Huang, X., Zheng, C., Liu, F., Lu, X, Tian, J., Chung, T., Otegui, M.S., Xiao, S., Vierstra, R.D., Li, F. 2019. Genetic analyses of the Arabidopsis Atg1 kinase complex reveals both kinase-dependent and independent autophagic routes during fixed-carbon starvation. Plant Cell 31: 2973-2995. PMID 31615848.

Paez-Valencia, J. and Otegui, M.S. Purification of plant ESCRT proteins for polyclonal antibody production. 2019. Methods in Molecular Biology 1998: 227-238.

Xiong, F., Ren, J.J., Yu, Q., Lu, C.C., Kong, L.J., Otegui, M.S., Wang, X.L. 2019. AtU2AF65b acts via ABI5 and FLC splicing to regulate flowering in Arabidopsis. New Phytologist 223(1):277-292. PMID: 30790290

Xiong, F., Ren. J., Yu, Q., Wang, Y., Kong, L.-J., Otegui, M.S., Wang, X.-L. 2019. Arabidopsis AtBUD13 is essential for embryo development via pre-mRNA splicing. Plant Journal 98(4):714-726. PMID: 30720904


Otegui, M.S. Pennington, J. 2018. Electron tomography in plant cell biology. Microscopy 68(1):69-79. PMID: 30452668

Ding, X., Zhang, X., Otegui, M.S. 2018. Plant autophagy: new flavors on the menu. Current Opinion in Plant Biology 26: 113-121. PMID: 30267997.

McLoughlin, F., Augustine, R., Marshall, R., Li, L., Kirkpatrick, L., Otegui, M.S., Vierstra, R.D. 2018. Integrated multi-omics analyses of maize reveal roles for autophagic recycling in proteome remodeling and lipid turnover. 2018. Nature Plants 4: 1056-1070. PMID: 30478358

Mishev, K., Lu, Q., Dejonghe, W., Denoo, B, Hullaert, J., Sharma, I., Nerinck, W., De Rycke, R., Goodman, K., Kalinowska, K., Storme, V., Boeren, S., Nguyen, L., Drozdzecki, A., Audenaert, D., Otegui, M.S., Isono, E., Madder, A., De Vries, S., Winne, J., and Russinova, E. 2018. Nonselective chemical inhibition of Sec7 domain-containing ARF GEFs in Arabidopsis. Plant Cell, 30: 2573-2593. PMID: 30018157

Paris, R., Vazquez, M.M, Graziano, M., Miller, N.D., Spalding, E.P., Otegui, M.S., and Casalongué, C.A. 2018. Dynamic distribution of endogenous NO modulates auxin-dependent PIN2 localization during early gravitropism. Frontiers in Plant Science 9: 495. PMID: 29731760

Otegui, M.S. 2018. ESCRT-mediated sorting and intralumenal vesicle concatenation in plants. Biochem Soc Trans, 46: 537-545. Invited review. PMID: 29666213.

Chanoca, A., Burkel, B., Grotewold, E., Eliceiri, K.W., Otegui, M.S. 2018. Imaging vacuolar anthocyanins with fluorescence lifetime microscopy (FLIM). Methods in Molecular Biology 1789:131-141 PMID: 29916076

Lee, H.N., Zarza, X., Kim, J.H., Yoon, M.J., Kim, S.H., Lee, J.H., Paris, N., Munnik, T., Otegui, M.S., Chung, T. 2018. Arabidopsis VPS38 is required for vacuolar trafficking but dispensable for autophagy. Plant Physiology, 176: 1559-1572. PMID: 29184027

Otegui, M.S. 2018. Vacuolar degradation of chloroplast components: autophagy and beyond. Journal of Experimental Botany 69 (4): 741-750. Invited review. PMID: 28992297


Ertel, K.J., Benefield D., Castano-Diez, D., Pennington, J., Horswill, M., den Boon, J.A., Otegui, M.S., and Ahlquist, P. 2017. Cryo-electron microscopy reveals novel features of a viral RNA replication compartment. eLife, in pressPMID: 28653620

Buono, R.A., Leier, A., Paez-Valencia, J., Pennington, J., Goodman, K., Miller, N., Ahlquist, P., Marquez-Lago, T., Otegui, M.S. 2017. ESCRT-mediated vesicle concatenation in plant endosomes. Journal Cell Biology 216: 2167-2177. PMID: 28592443

Cox, B. L., Schumacher, N., Konieczny, J., Reifschneider, I, Mackie, T., Otegui, M.S., Eliceiri, K.W. 2017. Fabrication approaches for the creation of physical models from microscopy data. 3D Printing in Medicine3:2


Huang, J., Zhang, T., Linstroth, L., Tillman, Z., Otegui, M.S., Owen, H., Zhao, D. 2016. Control of anther cell differentiation by the TPD1 small protein ligand and its receptor EMS1 in Arabidopsis. PLOS Genetics, 12(8):e1006147. PMID: 27537183

Chanoca, A., Burkel, B., Kovinich, N., Grotewold, E., Eliceiri, K.W., Otegui, M.S. 2016. Using fluorescence lifetime microscopy to study trafficking of anthocyanins. The Plant Journal 88: 895-903. PMID: 27500780

Buono, R., Paez Valencia, J., Miller, N.D., Spitzer, C., Spalding, E.P., Otegui, M.S. 2016. Role of SKD1 regulators LIP5 and IST1-LIKE 1 in endosomal sorting and plant development. Plant Physiology 171:251-264.

Paez-Valencia, J., Goodman, K., Otegui, M.S. 2016. Endocytosis and endosomal trafficking in plants. Annual Review of Plant Biology 67:309-35. PMID: 27128466


Stefano, G. Renna, L., Lai, Y. Slabaugh, E., Mannino, N., Buono, R.A., Otegui, M.S. Brandizzi, F. 2015. ER network homeostasis is critical for plant endosome streaming and endocytosis. Cell Discovery 1: 15033. PMID: 27462431.

Chanoca, A., Kovinich, N., Burkel, B., Stecha, S., Bohorquez- Restrepo, A., Ueda, T., Eliceiri, K., Grotewold, E., Otegui, M.S. 2015. Anthocyanin vacuolar inclusions form by a microautophagy mechanism. The Plant Cell, 27: 2545-59. PMID: 26342015. Featured on the cover. Commentary on Bassham, D. 2015. Pigments on the move. Nature 526(7575):644-5.

Kalinowska, K., Nagel, M.K., Goodman, K., Cuyas, L., Anzenberger, F., Alkhofer, A., Paz-Ares, J., Braun, P., Rubio, V., Otegui, M.S., Isono, E. 2015. Arabidopsis ALIX is required for the localization of the deubiquitinating enzyme AMSH3. PNAS, 112(40):E5543-51. PMID: 26324913

Naramoto, S., Dainobu, T., Otegui, M.S. 2015. A Bioimaging pipeline to show membrane trafficking regulators localized to the Golgi apparatus in plant cells. Bioprotocols 5(17): e1583.

Kovinich, N., Kayanja, G., Chanoca, A., Otegui, M.S., Grotewold, E. 2015. Abiotic stresses induce different localizations of anthocyanins in ArabidopsisPlant Signaling and Behavior 10(7):e1027850. PMID: 26179363.

Li, F., Chung, T., Pennington JG, Federico M.L., Kaeppler, H.F., Kaeppler, S.M., Otegui, M.S. Vierstra, R.D. 2015. Autophagic recycling plays a central role in maize nitrogen remobilization. The Plant Cell 27: 1389-408. PMID: 25944100

Reguera, M., Bassil, E., Tajima, H., Wimmer, M., Chanoca, A., Otegui, M.S., Paris, N., Blumwald, E. 2015. pH regulation by NHX-type endosomal antiporters is required for receptor-mediated protein trafficking to the vacuole. Plant Cell 27:1200-17. PMID: 25829439.

Spitzer, C. Li, F., Buono, R., Roschzttardtz, H., Chung, T., Zhang, M., Osteryoung, K.W., Vierstra, R.D., Otegui, M.S. 2015. The Endosomal Protein CHARGED MULTIVESICULAR BODY1 Regulates the Autophagic Turnover of Plastids in ArabidopsisThe Plant Cell 27:391-402. PMID: 25649438.

Johnson, A., Bhattacharya, N., Hanna, M., Pennington J.G., Schuh, A.L., Wang, L., Otegui, M.S., Stagg, S., Audhya, A. 2015. TFG facilitates COPII transport carrier clustering and early secretory pathway organization. EMBO Journal 34:811-27. PMID: 25586378

Velasquez, S.M., Ricardi, M.M., Poulsen, C., Oikawa, A., Dilokpimoladil, A., Halim, A., Mangano, S., Denita Juarez, S.P., Marzol, E., Salgado Salter, J.D., Gloazzo Dorosz, J., Borassi, C., Möller, S., Buono, R., Ohsawa, Y., Matsuoka, K., Otegui, M.S., Scheller, H.V., Geshi, N., Larsen Petersen, B., Iusem, N.D., Estevez, J.M. 2015. Key role of Prolyl-4-hydroxylase 5 on root hair cell expansion. Molecular Plant 8:734-46. PMID: 25655826.


Roschzttardtz, H., Paez Valencia, J., Dittakavi, T., Jali,S., Reyes, F.C., Baisa, G., Anne, P., Gissot, L., Palauqui, J.C., Masson, P.H., Bednarek,S.Y., Otegui, M.S. 2014. The VASCULATURE COMPLEXITY AND CONNECTIVITY (VCC) gene encodes a plant-specific protein required for embryo provasculature development. Plant Physiology 166: 889-902. PMID: 25149602.

Shen, Q.-T., Schuh, A.L. Zheng, Y., Mitchell, J.C., Otegui, M.S., Ahlquist, P., Cui, Q., Audhya, A. 2014. Structural analysis and modeling reveals new mechanisms governing ESCRT-III spiral filament assembly. Journal Cell Biology 206:763-77. PMID: 25202029

Naramoto, S., Otegui, M.S., Kutsuna, N., de Rycke, R., Dainobu, T., Karampelias, M., Fujimoto, M., Feraru, E., Miki, D., Fukuda, H., Nakano, A,. Friml, J. 2014. Insights into the localization and function of the membrane trafficking regulator GNOM ARF-GEF at the Golgi apparatus in Arabidopsis. The Plant Cell 26(7):3062-76.

Kovinich, N., Kayanja, G., Chanoca, A., Riedl, K., Otegui, M.S., Grotewold, E. 2014. Not all anthocyanins are born equal: Distinct patterns induced by stress in ArabidopsisPlanta 240: 931-940.

Reyes, F.C., Buono, R.A. Roschzttardtz, H., Di Rubbo, S., Yeun, L.H., Russinova,E. Otegui, M.S. 2014. A novel endosomal sorting complex required for transport (ESCRT) component in Arabidopsis thaliana controls cell expansion and development. Journal Biological Chemistry 289: 4980-4988.

Otegui, M. (Editor). Plant Endosomes, Methods in Molecular Biology, Humana Press. 2014. This is a book consisting of 20 chapters on protocols for the analysis of plant endosomes and endomembrane system.

Chanoca, A. Otegui, M.S. 2014. Immunogold labeling and electron tomography of endosomes. In Otegui, M.S. (ed) Plant Endosomes, Methods in Molecular Biology, Humana Press. PMID: 25117275

Otegui, M.S. 2014. Electron Tomography of Plant Cells. In: Assmann, S. and Liu, B. The Plant Sciences – Cell Biology.  Springer-Verlag, Heidelberg, Germany.


Liang, Z., Demko, V., Wilson, Robert, Johnson, K., Ahmad, R., Perroud, P.-F., Quatrano, R., Zhao, S.; Shalchian-Tabrizi, K., Otegui, M.S., Olsen, O.-A.; Johansen, W. 2013. The catalytic domain CysPc of the DEK1 calpain is functionally conserved in land plants. Plant Journal: 75:742-54.

Sauer, M., Otilia Delgadillo Lopez, M., Zouhar, J., Reynolds, G., Liljegren, S., Stierhof, Y., de Jaeger,G., Otegui,M.S., Bednarek,S., Rojo, E. 2013. MTV1 and MTV4 encode plant specific ENTH and ARF GAP proteins mediating clathrin-dependent trafficking of vacuolar cargo from the TGN. The Plant Cell, 25:2217-35.


Otegui, M.S., Buono, R., Reyes, F., Roschzttardtz, H. 2012. ESCRT-dependent sorting in late endosomes. In: Endocytosis in Plants, edited by J. Samaj. Springer-Verlag Berlin Heidelberg.


Reyes FC, Buono R, Otegui MS. 2011. Plant endosomal trafficking pathways. Curr. Opin. Plant Biol. 14: 666-73.

Capoen, W., Sun, J., Hirsch, S., Otegui, M.S., Venkateshwaran, M., Miwa, H., Downie, J.A., Ané, J.-M., Oldroyd, G.E.D. 2011. Nuclear membranes control symbiotic calcium signaling of legumes. Proc. Natl. Acad. Sci. USA. 108:14348-53.

Otegui, M.S. 2011. Electron tomography and immunogold labeling as tools to analyze de novo assembly of plant cell walls. Methods Mol. Biol. 715:123-40.

Reyes, F.C., Chung, T., Holding, D., Jung, R., Vierstra, R., Otegui, M.S. 2011. Delivery of prolamins to the protein storage vacuole in maize aleurone cells. Plant Cell 23:769-84. PMID: 2134341

Wu, G., Wang, X, Li, X., Kasahara, H., Kamiya, Y., Otegui, M.S. and Chory, J. 2011. Methylation of a phosphatase specifies dephosphorylation and degradation of activated brassinosteroid receptors. Science Signal. 4:ra29. PMID: 21558554.


Otegui, M.S., Reyes, F.C. 2010. Endosomes in plants. Nature Education 3(9): 23.

Wu, G., Otegui, M.S., Spalding, E.P. 2010. Trafficking of ABCB19, a plant multidrug resistance-like transporter required for auxin-mediated root cell elongation, requires the ER-localized TWD1 immunophilin. Plant Cell 22: 3295-3304.

Reyes FC, Sun B, Guo H, Gruis D, Otegui MS. 2010. Agrobacterium tumefaciens-mediated transformation of maize endosperm as a tool to study endosperm cell biology. Plant Physiol. 153: 624-631.

Haruta, M., Burch, H.L., Nelson, R.B., Barrett-Wilt G., Kline, K.G., Mohsin, S.B., Young, J.C., Otegui, M.S., Sussman, M. 2010. Molecular characterization of mutant Arabidopsis plants with reduced plasma membrane proton pump activity. J. Biol. Chem. 285:17918-29. PMID: 20348108.


Peer, W.A., Hosein, F.N., Bandyopadhyay, A., Makam, S.N., Otegui, M.S., Lee, G.J., Blakeslee, J.J., Cheng, Y., Titapiwatanakun, B., Yakubov, B., Bangari, B., Murphy, A.S. 2009. Mutation of the membrane-associated M1 protease APM1 results in distinct embryonic and seedling developmental defects in ArabidopsisPlant Cell 21:1693-721.

Spitzer, C., Reyes, F.C., Buono, R., Sliwinski, M.K., Haas, T.J., Otegui, M.S. 2009. The ESCRT-related CHMP1A and B proteins mediate multivesicular body sorting of auxin carriers and are required for plant development. Plant Cell 21: 749-766.

Stanga, J., Boonsirichai, K., Sedbrook, J., Otegui, M. Masson, P. 2009. A role for the TOC complex in Arabidopsis root gravitropism. Plant Physiol. 149:1896-905.

Otegui, M.S., Spitzer, C. 2008. Endosomal functions in plants. Traffic 9:1589-98.


Ebine, K. Okatani, Y., Uemura, T., Goh, T., Shoda, K., Niihama, M., Terao Morita, M., Spitzer, C., Otegui, M.S., Nakano, A. and Ueda, T. 2008. A SNARE complex unique to seed plants is required for vacuole biogenesis and seed development of Arabidopsis thaliana. Plant Cell 20: 3006-21

Martínez, D.E., Costa, M.L., Otegui, M.S., Guiamet, J.J. 2008. Senescence-associated vacuoles of tobacco leaves are involved in the degradation of chloroplast proteins. Plant J. 56: 196-206.

Bassham, D., Brandizzi, F., Otegui, M.S., Sanderfoot, A.A. 2008. The Secretory System of Arabidopsis. In: The Arabidopsis Book ISSN 1543-8120


Doelling, J.H., Soyler-Ogretim, G., Phillips, A.R., Wise, J., Chandler, J., Callis, J., Otegui, M.S., and Vierstra, R.D. 2007. The ubiquitin-specific protease subfamily UBP3/UBP4 is essential for pollen development and transmission in Arabidopsis thaliana. Plant Physiol. 145: 801-13.

Tian, Q., Olsen, L., Sun, B., Lid, S.E., Brown, R., Lemmon, B.E., Fosnes, K., Gruis, F., Opsahl-Sorteberg, H.-G., Otegui, M.S. and Olsen, O.-A. 2007. Subcellular localization and functional domain studies of Defective Kernel 1 in maize and Arabidopsis thaliana suggest a model for aleurone cell fate specification involving CRINKLY 4 and SUPERNUMERARY ALEURONE LAYER 1. Plant Cell 19: 3127-3145.

Haas, T.J., Otegui, M.S. 2007. Electron tomography in plant cell biology. J. Integr. Plant Biol. 49: 1091-1099.

Holding, D.R., Otegui, M.S., Li, B., Meeley, R., Dam, T., Hunter, B.G., Jung, R., Larkins, B.A. 2007. The maize Floury1 gene encodes a novel ER protein involved in zein protein-body formation. Plant Cell 19: 2569-2582.

Peiter, E., Sun, J., Heckmann, A.B.,  Venkateshwaran, M., Riley, B.K.,  Otegui, M.S., Edwards, A., Freshour, G., Hahn, M.G.,  Cook, D.R., Sanders, D.,  Oldroyd, G.E.D., Downie, J.A.,  Ané, J.-M. 2007 The Medicago truncatula DMI1 protein modulates cytosolic calcium signaling. Plant Physiol. 145: 192-203.

Kobayashi, K, Otegui, M.S., Krishnakumar, S., Mindrinos, M., Zambryski, P. 2007. INCREASED SIZE EXCLUSION LIMIT 2 encodes a DEVH box RNA helicase that regulates plasmodesmata during Arabidopsis embryogenesis. Plant Cell 19: 1885-1897.

Haas, T.J., Sliwinski, M.K., Martínez, D.E., Preuss, M., Ueda, T., Nielsen, E., Odorizzi, G., and Otegui, M.S. 2007. The Arabidopsis AAA ATPase SKD1 is involved in multivesicular endosome function and interacts with its positive regulator LIP5. Plant Cell 19:1295-312. PMID: 17468262.

Seguí-Simarro, J.M., Otegui, M.S., Austin, J.R. II, Staehelin, L.A. 2007.  Plant cytokinesis – Insights gained from electron tomography studies. In: Verma, D.P.S. and Hong, Z., Cell Division Control in Plants. Springer-Verlag, Heidelberg, Germany.

Otegui, M.S. 2007. Endosperm cell walls: formation, composition, and functions. In: Olsen, O.-A. Endosperm: development and molecular biology. Springer-Verlag, Heidelberg, Germany.

Otegui, M.S., Austin, J.R. II. 2007. Visualization of membrane-cytoskeletal interactions during plant cytokinesis. Methods Cell Biol. 79:221-40.


Otegui, M.S., Herder, R., Schulze, J., Jung, R., Staehelin, L.A. 2006. The proteolytic processing of seed storage proteins in Arabidopsis embryo cells starts in the multivesicular bodies. Plant Cell 18: 2567-2581.

Chaiwongsar, S., Otegui, M.S., Jester, P.J., Monson, S.S., Krysan, P.J. 2006. The protein kinase genes MAP3K1 and MAP3K2 are required for pollen viability in Arabidopsis thalianaPlant J. 48:193-205.

Petruccelli, S., Otegui, M.S., Lareu, F., Trandinhthanhlien, O., Fitchette, A.-C., Circosta, A., Rumbo, M., Bardor, M., Carcamo, R., Gomord, V., Beachy, R. 2006. A KDEL tagged monoclonal antibody is efficiently retained in the reticulum endoplasmic in leaves but is both partially secreted and sorted to protein storage vacuoles in seeds.  Plant Biotech. J. 4: 511-527.

Preuss, M.L., Schmitz, A.J., Thole, J.M. Bonner, H.K.S., Otegui, M.S., Nielsen, E. 2006. A role for the RabA4b effector protein, PI-4Kβ1, in polarized expansion of root hair cells in ArabidopsisJ. Cell Biol. 172:991-8.


Otegui, M.S., Noh, Y.S., Martínez, D.E., Vila Petroff, M. G., Staehelin, L.A., Amasino, R.M., Guiamet, J.J. 2005. Senescence-associated vacuoles with intense proteolytic activity develop in leaves of Arabidopsis and soybean. Plant J. 41: 831-844.

Otegui,M.S., Verbrugghe, K.J., Skop, A.R. 2005. Midbodies and phragmoplasts; analogous structures involved in cytokinesis. Trends Cell Biol. 15: 404-13.


Otegui, M.S., Staehelin, L.A. 2004. Electron tomographic analysis of post-meiotic cytokinesis during pollen development in Arabidopsis thalianaPlanta 218: 501 – 515


Otegui, M.S., Capp, R., Staehelin, L.A. 2002. Developing seeds of Arabidopsis thaliana store different minerals in two types of vacuoles and in the ER. Plant Cell 14: 1311-1327.


Mineyuki, Y., Karahara, I., Murata, T. Otegui, M.S., Giddings, T.H., Staehelin, L.A. 2001. High pressure freezing in plant tissue. Electron Microscopy 36: 105-107.

Otegui, M.S., Mastronarde, D., Kang, B-H., Bednarek, S.Y., Staehelin, L.A. 2001. Three-dimensional analysis of syncytial type cell plates during endosperm cellularization visualized by high resolution electron microscopy. Plant Cell 13: 2033-2051.


Otegui, M.S., Staehelin, L.A. 2000. Syncytial-type cell plates. A novel kind of cell plate involved in endosperm cellularization of Arabidopsis thalianaPlant Cell 12: 933-947.

Otegui, M.S., Staehelin, L.A. 2000. Cytokinesis in flowering plants: more than one way to divide a cell. Curr. Opin. Plant Biol. 3: 493-502.


Otegui, M.S., Cocucci, A. 1999. Flower morphology and biology of Myrsine laetevirens, structural and evolutionary implications of anemophily in Myrsinaceae. Nordic J. Botany 19: 71-85.

Otegui, M.S., Maldonado, S., Lima, C., Lederkremer, R. 1999. Development of the endosperm of Myrsine laetevirens (Myrsinaceae): II. Formation of the protein and lipid bodies. Inter. J. Plant Sci. 160: 501-509.

Otegui, M.S., Lima, C., Maldonado, S., Lederkremer, R. 1999. Development of the endosperm of Myrsine laetevirens (Myrsinaceae): I. Cellularization and deposition of cell wall storage. Inter. J. Plant Sci. 160: 491-500.

Otegui, M.S., Lima, C., Maldonado, S., Lederkremer, R. 1998. Histological and chemical characterization of Myrsine laetevirens seed. Inter. J. Plant Sci.159: 762-772.

Maldonado, S., Otegui, M.S., Bozini, J. P. 1998. Embryological studies in Sanango racemosumNordic J. Botany 18: 599-609.

Otegui, M.S., Gaspar, M. L., Maldonado, S., Varetti, E. L. & Pollero, R. 1998. Studies on tissues associated to hydroxybenzoquinone secretion in Myrsine laetevirens (Myrsinaceae). Nordic J. Botany 18: 447-459.

Otegui, M.S., Maldonado, S. 1998. Embryological features and bacterial population in the gynoecium of Myrsine laetevirensActa Bot. Neerlandica 47: 185-194.

Prego, I, Maldonado, S., Otegui, M.S. 1998. Studies on quinoa seed: Seed Structure and Localization of Seed Reserves. Annals Botany 82: 481-488

Maldonado, S., Otegui, M.S. 1997. Secretory tissues of the flower of Sanango racemosum (Gesneriaceae). I. Light microscopy. Acta Bot. Neerlandica 46: 413-420.

Otegui, M.S. 1994. Occurrence of perforated ray cells and ray splitting in Rapanea laetevirens and R.lorentziana (Myrsinaceae). I.A.W.A. Journal 15: 257-263.